| ¤ Appearance |
crystalline powder |
| ¤ Color |
white to light-creamy |
| ¤ Taste |
bitter |
| ¤ Odor |
odorless |
| ¤ Melting temperature (ºC) |
approximately 240.0 (with disintegration) |
 successive melting temperatures
(ºC) |
155.0; 204.0; 212.0 |
| ¤ Solubility |
|
| in water (20ºC) |
poorly soluble |
| in alcohol 20ºC) |
poorly soluble |
| in methanol (20ºC) |
poorly soluble |
| in hot alcohol |
soluble |
| in hot methanol |
soluble |
| in hot pyridine |
soluble |
| in hot dioxane |
soluble |
| ¤ Aqueous and alcoholic
solutions are colored in blue fluorescence. Readily and
quickly hydrolyzed in acid medium to Esculin and glucose. |
| ¤ Moisture content according to K. Fisher
(%) |
6.50 - 8.0 |
| ¤ Sulfate ash (%) |
not more than 0.1 |
| ¤ Heavy metals content (%) |
not more than 0.004 |
| ¤ Limpidity of solution |
need not meet standard No 5 |
¤ Specific rotation (º)
(2% solution of anhydrous esculin (wt/v) in 50%
dioxane solution) |
from -84.0 to -87.0 |
¤ Extinction coefficient (µm)
(0.0015% solution in methanol) |
from 0.340 to -0.360 |
| ¤ Particle size (µm) |
min 95.0 - 180.0 |
| ¤ Esculin content calculated as dry
substance |
not less than 98.0 |
| ¤ Microbial content |
|
| total microorganisms (number/g) |
not more than 10 -3 |
| moulds and yeast (number/g) |
not more than 10 -2 |
| Enterobacteriaceae, Ps.
aerguinosa, St. aureus |
not allowed |
